ISS Inc

05/29/2026

A study in the Journal of Human Kinetics investigated how rest redistribution—breaking long rest periods into shorter, more frequent ones—affects fatigue during maximal eccentric knee extensions. 💪

To monitor muscle oxygenation throughout the exercise protocol, researchers relied on the ISS OxiplexTS, a powerful NIRS device that delivers real-time insights into tissue oxygenation and hemodynamics. 📈

The study found that rest redistribution influences fatigue and muscle performance, offering valuable insights for athletic training and rehabilitation strategies.

📖 Read the full publication: https://pmc.ncbi.nlm.nih.gov/articles/PMC7706641/

➡️Check out the OxiplexTS: https://iss.com/biomedical/oxiplexts

If your research involves muscle oxygenation, oxygen kinetics, exercise physiology, or sports medicine we'd love to show you what this data actually looks like in practice.

Come find us at Booth #716. No pressure, no sales pitch. Just a quick look at the technology and a conversation about your work.

📍 Salt Lake City Convention Center
📅 May 26–29, 2026
🔬 ISS Medical Booth #716

05/28/2026

The question we've heard most at Booth #716 so far: "What does the data actually look like?"

Here's the short answer: it looks like a live, real-time trace of oxygenated and deoxygenated hemoglobin in your target tissue, updating at up to 50 times per second.

Unlike conventional NIRS devices, the OxiplexTS uses frequency-domain photon migration to independently measure both light absorption AND scattering in the tissue. That's what makes it possible to report absolute concentrations, not just relative changes from an arbitrary baseline.

For researchers, that distinction matters for:

→ Between-subject comparisons
→ Multi-site and longitudinal studies
→ Grant applications where reviewers want methodology rigor
→ Publications in high-impact journals where limitations sections get scrutinized

We have two days left on the exhibit floor. If you haven't stopped by yet...Booth #716, Salt Lake City Convention Center. The system is on.

📅 Open through Friday, May 29

05/27/2026

We're live at ACSM 2026!
Day one on the exhibit floor. Booth #716 is open, the system is running, and we're ready to talk tissue oxygenation with anyone who wants to stop by.

This year's ACSM spotlight — Physical Activity, Exercise & Technology — could not be a better fit for what we do. Frequency-domain near-infrared spectroscopy is exactly the kind of advanced measurement technology that's pushing exercise science forward: non-invasive, quantitative, and validated across 150+ peer-reviewed studies.

If you're here in Salt Lake City and want to see what absolute muscle oxygenation data looks like in real time — no relative changes, no assumptions, no calibration — come by between sessions.

We'll keep it brief. You'll leave with a clear sense of whether this fits your research.

📍 ISS Medical · Booth #716
📅 Today through Friday, May 29

05/26/2026

SPORTS MEDICINE RESEARCH WITH OXIPLEXTS
The OxiplexTS, a non-invasive near-infrared tissue oximeter, supports a range of research applications in Sports Medicine. In this publication, it was used to investigate whether athletes, such as cyclists, with high-affinity hemoglobin (HAH) exhibit reduced skeletal muscle deoxygenation during exercise under both normoxic and hypoxic conditions.

Read the full publication here: https://physoc.onlinelibrary.wiley.com/doi/epdf/10.1113/EP090308

Additional OxiplexTS publications: https://iss.com/biomedical/oxiplexts

If your research involves muscle oxygenation, oxygen kinetics, exercise physiology, or sports medicine we'd love to show you what this data actually looks like in practice.

Come find us at Booth #716. No pressure, no sales pitch. Just a quick look at the technology and a conversation about your work.

📍 Salt Lake City Convention Center
📅 May 26–29, 2026
🔬 ISS Medical Booth #716

05/22/2026

05/22/2026

Most performance tools measure what an athlete does. OxiplexTS measures what's happening inside the muscle while they do it.

Heart rate. Power output. GPS. These metrics tell you the result of an effort but not the physiological story behind it.

Near-infrared tissue oximetry fills that gap. By measuring oxygenated and deoxygenated hemoglobin directly in the target tissue in real time, non-invasively, and without calibration assumptions. The OxiplexTS gives researchers and sports medicine professionals a window into muscle oxygen delivery and utilization that no other tool provides.

What that means in practice:

→ See the exact moment a muscle shifts from aerobic to anaerobic during exercise, before lactate rises, before output drops
→ Track post-exercise oxygen recovery as an objective marker of readiness, not a subjective wellness score
→ Compare data across subjects, sessions, and sites with absolute measurements that don't depend on a baseline assumption
→ Support return-to-sport decisions with a non-invasive physiological measurement that goes beyond what functional performance tests alone can tell you

This is the technology behind 150+ peer-reviewed studies in exercise physiology, sports medicine, and human performance research.

If you're attending ACSM 2026 in Salt Lake City next week come find us at Booth #716. We'll have a live, functioning system on-site and we'd love to show you what this data looks like in real time.

No appointment. No pressure. Just the science.

📍 Booth #716 | Salt Lake City Convention Center
📅 May 26–29, 2026
🔗 https://iss.com/biomedical

05/22/2026

What happens when two molecular species move through the same space but emit similar fluorescence signals? Traditional correlation methods may struggle to separate them. FLCS changes that.

📩 Exploring FLCS, TTTR acquisition, or multi-species diffusion analysis? Let’s talk: https://iss.com/contact

The data shown here demonstrates Fluorescence Lifetime Correlation Spectroscopy (FLCS) of a mixture containing:

🟢 Alexa 647 (free in solution)
🔴 Atto 647N (attached to a small functional unit)

Although both fluorophores occupy the same environment, their distinct fluorescence lifetimes allow ISS FLCS workflows to separate and analyze each population independently.

How does FLCS work? ISS collects FCS data in TTTR (Time-Tagged Time-Resolved) mode, generating both:

📊 Correlation curves
⏱️ Fluorescence decay curves

The decay profile is fit to determine the characteristic lifetimes of each species. In this example:

• Alexa 647 → 0.975 ns
• Atto 647N → 3.644 ns

These lifetime signatures are then used to build lifetime filters, which assign weighting factors to separate photons originating from:

✔️ Alexa 647
✔️ Atto 647N
✔️ Background signal

Once separated, independent correlation curves are generated for each species and fit to determine their individual diffusion dynamics.

Why does FLCS Matter? FLCS extends traditional FCS by adding a new dimension of contrast: fluorescence lifetime. This enables researchers to:

✔️ Separate overlapping fluorescent populations
✔️ Reduce background influence
✔️ Analyze multiple dynamic species simultaneously
✔️ Improve confidence in diffusion measurements
✔️ Study heterogeneous biological systems with greater precision

Applications include:

• Protein interactions
• Molecular transport
• Binding studies
• Complex mixtures
• Live-cell fluctuation analysis

Advanced Analysis with VistaVision. Enhanced by Time-Tagging Innovation Through its collaboration with Swabian Instruments. Find out more here: https://www.swabianinstruments.com/iss-swabian-instruments-collaboration/

ISS platforms support FLCS acquisition through:

✔️ FastFLIM (digital frequency-domain acquisition)
✔️ Digital TCSPC acquisition
✔️ TTTR data collection
✔️ Integrated VistaVision FLCS routines
✔️ Direct connection to the VistaVision FFS fitting module

This enables researchers to move seamlessly from lifetime separation → correlation analysis → diffusion quantification.

Combining confocal imaging, FLIM, spectroscopy, and fluorescence fluctuation analysis into a unified quantitative workflow.

💬 Bottom line:
ISS FLCS transforms fluorescence lifetime information into cleaner separation, more accurate diffusion measurements, and deeper insight into molecular behavior.

05/21/2026

We're heading to Salt Lake City for ACSM 2026 and we're bringing the science with us. The 2026 ACSM Annual Meeting spotlight is Physical Activity, Exercise & Technology. For us, that's not a theme. It's what we've been building for decades.

ISS will be exhibiting at Booth #716 with a live, functioning OxiplexTS, the only commercially available frequency-domain near-infrared spectroscopy system that delivers absolute, non-invasive tissue oxygenation measurements without calibration assumptions.

If your research involves muscle oxygenation, oxygen kinetics, exercise physiology, or sports medicine then we'd love to show you what this data actually looks like in practice.

Come find us at Booth #716. No pressure, no sales pitch. Just a quick look at the technology and a conversation about your work.

📍 Salt Lake City Convention Center
📅 May 26–29, 2026
🔬 ISS Booth #716

05/21/2026

Separating Real Interactions from Spectral Crosstalk with PIE-FCCS. Two-color fluorescence experiments can reveal molecular interactions but only if you can trust the correlation signal.

📩 Working on FCCS, interaction studies, or spectral crosstalk challenges? Reach out here: https://iss.com/contact

One of the biggest challenges in two-color Fluorescence Cross-Correlation Spectroscopy (FCCS) is distinguishing true molecular co-diffusion from false cross correlation caused by spectral bleedthrough.

The data shown here demonstrates PIE-FCCS (Pulsed Interleaved Excitation Fluorescence Cross-Correlation Spectroscopy) and gating-FCCS processing using a mixture of Cy3B and Atto 647N dyes freely diffusing in water.

Initially, a measurable cross correlation appeared. Not because the dyes interacted, but because Cy3B photons leaked into the Atto 647N detection channel, creating a false positive signal.

ISS solves this challenge using Pulsed Interleaved Excitation (PIE).

By temporally separating excitation events, the fluorescence decays of each fluorophore become offset in time. VistaVision then applies gating-FCCS processing to isolate only the photons belonging to each fluorophore.

The result:

✔️ Removal of spectral crosstalk artifacts
✔️ Elimination of false cross correlation
✔️ More reliable interpretation of molecular interaction data

Why PIE-FCCS Matters. Two-color FCCS extends traditional FCS by measuring:

📊 Autocorrelation of fluorophore A
📊 Autocorrelation of fluorophore B
📊 Cross correlation between both species

This enables quantitative studies of:

✔️ Protein-protein interactions
✔️ Receptor-ligand binding
✔️ Molecular complex formation
✔️ Co-diffusion behavior in live systems

With ISS, researchers gain the confidence that measured interactions reflect biology not optical artifacts.

Advanced Time-Resolved Detection with ISS

ISS platforms support PIE-FCCS acquisition using:

✔️ FastFLIM (digital frequency-domain acquisition)
✔️ Digital TCSPC acquisition
✔️ TTTR (Time-Tagged Time-Resolved) data collection
✔️ Tunable PIE configurations for multiple laser architectures
✔️ Synchronized gating detection optimized for:
• FLIM
• FCCS
• smFRET

This creates a highly flexible workflow for advanced fluorescence fluctuation studies.

💬 Bottom line:
ISS PIE-FCCS and gating analysis help researchers move beyond apparent correlations to uncover true molecular interactions with confidence.

05/20/2026

Salt Lake City sits at an average of 4,300 feet. Your research is already in altitude territory before the conference begins. So is ours. There's something fitting about bringing a tissue oximetry system to one of the highest-elevation cities in the U.S., a place where oxygen delivery and utilization are measurably different from sea level.

In this post we attempt to understand if, and by which means, humans fatigue differently when exposed to high-altitude and investigated any adaptations that occur with prolonged exposure at such altitude.

The muscle near-infrared spectroscopy (mNIRS) signals were recorded using the OxiplexTS tissue oximeter, which employed eight near-infrared light sources emitting at 690 and 830 nm. These emitters were positioned at varying distances from the detector to measure the absolute concentrations of oxygenated, deoxygenated, and total hemoglobin. The tissue oxygenation index (TOI) indicated the balance between oxygen supply and consumption. The mNIRS probe was secured over the re**us femoris muscle and sampled signals at 2 Hz from 1 minute before baseline testing until the end of the protocol.

Read the full publication here: https://journals.lww.com/acsm-msse/fulltext/2020/01000/high_altitude_acclimatization_improves_recovery.18.aspx

If you're presenting research at that involves tissue oxygenation, muscle metabolism, or oxygen kinetics. Stop by Booth #716 and let's talk about your protocol. We have a live system onsite and a team ready to answer your questions.

📍 Booth #716 | Salt Lake City Convention Center
📅 May 26–29, 2026

05/20/2026

Seeing Molecular Dynamics Across Space and Time with Scanning FCS. Understanding molecular mobility inside complex biological environments requires more than measuring a single point.
You need to see how diffusion changes across space, while preserving the dynamics over time.

📩 Exploring molecular mobility, condensates, or fluorescence fluctuation spectroscopy? Let’s talk: https://iss.com/contact

The data shown here demonstrates Scanning Fluorescence Correlation Spectroscopy (Scanning FCS) with Carpet Analysis of protein diffusion inside a protein-formed droplet.

In this experiment, FCS data was collected continuously for 300 seconds across 100 measurement points distributed along a 2 μm orbital scan. Rather than measuring a single location, ISS captures dynamic information across an entire spatial region simultaneously.

The result is visualized as a Carpet plot where:

📍 X-axis = measurement position along the orbit
⏱️ Y-axis = time progression

This representation transforms fluorescence fluctuations into an intuitive view of molecular behavior across both space and time.

Why Scanning FCS + Carpet Analysis Matters...Complex biological systems are rarely uniform. Molecular diffusion can vary significantly across compartments, condensates, membranes, and phase-separated environments.

ISS Scanning FCS enables researchers to:

✔️ Measure diffusion at multiple positions simultaneously
✔️ Observe spatial heterogeneity in molecular motion
✔️ Reduce photobleaching compared with stationary FCS
✔️ Capture long-duration measurements in live specimens

Using VistaVision’s integrated Carpet Analysis module, users can select regions directly from the Carpet for quantitative analysis.

In this example:

📊 Data from positions 50–57 were grouped together to calculate a diffusion curve, which was then fit in the VistaVision FFS fitting module to estimate the diffusion rate of Alexa Fluor™ 488-labeled proteins within the droplet.

ISS supports a complete family of Fluorescence Fluctuation Spectroscopy (FFS) methods, including:

✔️ FCS
✔️ Scanning FCS
✔️ FCCS with PIE
✔️ FLCS
✔️ PCH
✔️ RICS
✔️ Number & Brightness (N&B)

enabling researchers to connect molecular mobility, interaction, concentration, and lifetime behavior within one integrated workflow.

These advanced Scanning FCS workflows are supported across ISS confocal platforms including:

Alba v5
Alba STED
FLIMsight PRO
VIVIsight PRO
Q2

Supporting studies in protein dynamics, phase separation, intracellular transport, and molecular interactions.

💬 Bottom line:
ISS Scanning FCS and Carpet Analysis transform fluorescence fluctuations into high-resolution maps of molecular diffusion across both space and time.